1988•Laboratory AnimalsOpen access

An immunogold cell labelling method for rat T lymphocytes

G. O. Evans, Ruth M. Flynn, J. D. Lupton

Abstract

A method using monoclonal antibodies in conjunction with an immunogold procedure was adapted for labelling T lymphocytes in blood samples obtained from male Wistar rats. The monoclonal antibodies W3/25, MRC/OX8 and W3/13 were used. Changes in the percentages of positively labelled cells were observed in rats dosed with the immunosuppressant cyclosporin.

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Available abstract

Key concepts: Immunogold labelling, Labelling, Monoclonal antibody, Molecular biology, Biology, Monoclonal, Antibody, Immunology