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1956•Journal of Histochemistry & CytochemistryOpen access

HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES. IV. SOLUBLE OXIDATION-REDUCTION DYES AS AIDS IN THE HISTOCHEMICAL LOCALIZATION OF OXIDATIVE ENZYMES WITH TETRAZOLIUM SALTS

Emmanuel Farber, CONNIE D. LOUVIERE

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Abstract

1. In tetrazolium stains for the succinic dehydrogenase system, diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase, the rate of staining can he greatly increased by adding any one of a number of soluble oxidation-reduction indicators to the incubation medium. The characteristic staining patterns are not altered. 2. This modification of tetrazolium staining interposes a dye as a carrier between the enzyme and the tetrazolium salt and makes it possible to stain for enzymes which do not react directly with tetrazolium.

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1. In tetrazolium stains for the succinic dehydrogenase system, diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase, the rate of staining can he greatly increased by adding any one of a number of soluble oxidation-reduction indicators to the incubation medium. The characteristic staining patterns are not altered. 2. This modification of tetrazolium staining interposes a dye as a carrier between the enzyme and the tetrazolium salt and makes it possible to stain for enzymes which do not react directly with tetrazolium.

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Available abstract

1. In tetrazolium stains for the succinic dehydrogenase system, diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase, the rate of staining can he greatly increased by adding any one of a number of soluble oxidation-reduction indicators to the incubation medium. The characteristic staining patterns are not altered. 2. This modification of tetrazolium staining interposes a dye as a carrier between the enzyme and the tetrazolium salt and makes it possible to stain for enzymes which do not react directly with tetrazolium.

Key concepts: Formazan, Staining, Diaphorase, Oxidative enzyme, Enzyme, Biochemistry, Oxidative phosphorylation, Succinate dehydrogenase

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HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES. IV. SOLUBLE OXIDATION-REDUCTION DYES AS AIDS IN THE HISTOCHEMICAL LOCALIZATION OF OXIDATIVE ENZYMES WITH TETRAZOLIUM SALTS — Research Paper | ScholarLens