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Study on determination of biofilm activity in BAF by TTC-dehydrogenase assay

Zhan De-ming

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Abstract

The biofilms activity in Biological Aerated Filters(BAF) is quantitatively determined by 2,3,5-triphenyl tetrazolium chloride(TTC)-dehydrogenase assay and some major factors influencing the determination are studied in this paper.The optimum parameters are shown as follows:Tris-HCl buffers and 8.0 of pH,0.4%(mass fraction) of TTC and 0.1 mol/L of dextrose solution.The incubation time and temperature are 4 h and 40℃,respectively.The formazan reduction product can be efficiently extracted by methylbenzene,which can reduce the disturbance of dye color.This study demonstrates that the TTC-dehydrogenase assay can be successfully applied to the determination of biofilms activity in BAF.

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What this paper is about

The biofilms activity in Biological Aerated Filters(BAF) is quantitatively determined by 2,3,5-triphenyl tetrazolium chloride(TTC)-dehydrogenase assay and some major factors influencing the determination are studied in this paper.The optimum parameters are shown as follows:Tris-HCl buffers and 8.0 of pH,0.4%(mass fraction) of TTC and 0.1 mol/L of dextrose solution.The incubation time and temperature are 4 h and 40℃,respectively.The formazan reduction product can be efficiently extracted by methylbenzene,which can reduce the disturbance of dye color.This study demonstrates that the TTC-dehydrogenase assay can be successfully applied to the determination of biofilms activity in BAF.

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Available abstract

The biofilms activity in Biological Aerated Filters(BAF) is quantitatively determined by 2,3,5-triphenyl tetrazolium chloride(TTC)-dehydrogenase assay and some major factors influencing the determination are studied in this paper.The optimum parameters are shown as follows:Tris-HCl buffers and 8.0 of pH,0.4%(mass fraction) of TTC and 0.1 mol/L of dextrose solution.The incubation time and temperature are 4 h and 40℃,respectively.The formazan reduction product can be efficiently extracted by methylbenzene,which can reduce the disturbance of dye color.This study demonstrates that the TTC-dehydrogenase assay can be successfully applied to the determination of biofilms activity in BAF.

Key concepts: Formazan, Tetrazolium chloride, Chemistry, Dehydrogenase, Chromatography, Chloride, Tris, Colorimetry

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