1989PubMedRequires access

[Unification of the staining of histological preparations and histoautoradiograms with Harris hematoxylin].

Levdik Ti

Open publisher page 2 citations

Abstract

The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.

About this research paper

What this paper is about

The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.

Key concepts: Haematoxylin, Staining, Eosin, Fixation (population genetics), Fixative, H&E stain, Pathology, Stain

Related papers

Back to paper searchBrowse research topicsOriginal source
[Unification of the staining of histological preparations and histoautoradiograms with Harris hematoxylin]. — Research Paper | ScholarLens