[Unification of the staining of histological preparations and histoautoradiograms with Harris hematoxylin].
Levdik Ti
Abstract
Levdik Ti
Abstract
The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.
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The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.
Key concepts: Haematoxylin, Staining, Eosin, Fixation (population genetics), Fixative, H&E stain, Pathology, Stain