A Study on Tissue Processing

Histology, the study of tissue had been done for century in order to detect and observe normal structure of tissue. However, there are several reasons that might alter tissue from its original structure. Several processes, such as fixation, processing, embedding, cutting and staining had been done to reduce the limitation of tissue observation. In this study, ileum, skin, and appendix were experimental by using neutral buffered formaldehyde as fixative and stained with Van Gieson stain, Haematoxylin and eosin, Masson’s Trichrome stain and Periodic Acid Schiff.