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[Molecular typing of staphylococcus aureus with randomly amplified polymorphic DNA].

Ji Zhou, Sumei Guo

Abstract

The randomly amplified polymorphic DNA(RAPD) was used to type the 26 clinically isolated strains of staphylococcus aureus. The results were that all three primers of this method, ERIC-2, Ap1, and Ap7 on molecular typing of staphylococcus aureus and identified 9, 6, and 10 genotypes, respectively; the primer Ap7 produced better results than the other primers. The present study suggests that RAPD is a simple and rapid method and has a practical value in the molecular typing of staphylococcus aureus.

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Key concepts: Staphylococcus aureus, RAPD, Typing, Biology, Microbiology, Primer (cosmetics), DNA, Genotype