2010•Chinese Journal of Health Laboratory TechnologyRequires access

Role of RAPD for Pseudomonas aeruginosa typing

Yunjian Hu

Abstract

Objective:To investigate the role of random amplified polymorphic DNA for bacterial typing.Methods:The genomic DNA from 23 clinical strains of Pseudomonas aeruginosa was obtained,the genotypes of which were identified by random amplified polymorphic DNA assay with two random primers.Results:23 clinical strains of Pseudomonas aeruginosa could be divide into different genotypes with two random primers.Conclsions:It′s doubtful for the results of genotypes by random amplified polymorphic DNA with just one primer.

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Key concepts: RAPD, Pseudomonas aeruginosa, Primer (cosmetics), Genotype, Biology, genomic DNA, Typing, DNA