ScholarLens Back to search
1981•PubMedRequires access

Lymphokine (interleukin-2) production by mitogen-stimulated human lymphocytes in small reactors.

Bödeker Bg, Jörg Lehmann, Mühlradt Pf

Open publisher page 4 citations

Abstract

Human spleen cells or peripheral blood lymphocytes (PBL) were stimulated with the T-cell mitogens concanavalin A or phytohemagglutinin to produce the lymphokine interleukin-2 (IL-2). Culture conditions were optimized using 250-1000 ml reactor vessels. Optimal IL-2 release was found at a low speed of stirring allowing circulation of the medium without suspending the agglutinated cells, and at an oxygen concentration of about 30-45% of saturation. Cell viability was suboptimal at these conditions.

About this research paper

What this paper is about

Human spleen cells or peripheral blood lymphocytes (PBL) were stimulated with the T-cell mitogens concanavalin A or phytohemagglutinin to produce the lymphokine interleukin-2 (IL-2). Culture conditions were optimized using 250-1000 ml reactor vessels. Optimal IL-2 release was found at a low speed of stirring allowing circulation of the medium without suspending the agglutinated cells, and at an oxygen concentration of about 30-45% of saturation. Cell viability was suboptimal at these conditions.

Why it matters

OpenAlex reports 4 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Human spleen cells or peripheral blood lymphocytes (PBL) were stimulated with the T-cell mitogens concanavalin A or phytohemagglutinin to produce the lymphokine interleukin-2 (IL-2). Culture conditions were optimized using 250-1000 ml reactor vessels. Optimal IL-2 release was found at a low speed of stirring allowing circulation of the medium without suspending the agglutinated cells, and at an oxygen concentration of about 30-45% of saturation. Cell viability was suboptimal at these conditions.

Key concepts: Lymphokine, Concanavalin A, Interleukin 2, Mitogen-activated protein kinase, Chemistry, Saturation (graph theory), Interleukin, Immunology

Related papers

Related paper 1Related paper 2
Back to paper searchBrowse research topicsOriginal source
Lymphokine (interleukin-2) production by mitogen-stimulated human lymphocytes in small reactors. — Research Paper | ScholarLens