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AN IMPROVED ASSAY FOR INTERLEUKIN 2 (LYMPHOCYTE GROWTH FACTOR) PRODUCED BY MITOGEN‐ACTIVATED LYMPHOCYTES

Lafferty Kj, SJ Prowse, Abdul R. Al-Adra, HS Warren, J Vasalli, E. Reich

Open publisher page 54 citations

Abstract

The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.

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What this paper is about

The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.

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OpenAlex reports 54 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.

Key concepts: Concanavalin A, Lymphokine, Interleukin 2, Lymphocyte, Mitogen-activated protein kinase, Immunology, Molecular biology, Growth factor

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AN IMPROVED ASSAY FOR INTERLEUKIN 2 (LYMPHOCYTE GROWTH FACTOR) PRODUCED BY MITOGEN‐ACTIVATED LYMPHOCYTES — Research Paper | ScholarLens