2005•PubMedRequires access

[The inhibitory effects of siRNA expression vector on the expression of human papillomavirus E6 gene].

LI Da-ke, Zhilan Peng, Xiaoyu Niu, Danqing Wang

Abstract

OBJECTIVE: To investigate the inhibitory effects of RNAi(RNA interference)expression vector on the expression of human papilomavirus E6 gene. METHODS: siRNA expression vectors were constructed to be aimed directly at HPV16 E6 gene. The recombinants were transfected into cervical cancer cell line, caski, with liposomes. Expression of E6 was detected with fluorescence quantitative PCR (FQ-PCR) and flow cytometry. RESULTS: Three kinds of expression vectors could reduce the expression of E6 mRNA and protein all in caski-B cell. The expression of E6 mRNA reduced to 21.7% of control group, and the protein inhibition ratio reached 98.1%. CONCLUSION: The RNAi expression vector can effectively inhibit the expression of HPVE6 gene.

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Available abstract

Key concepts: RNA interference, Molecular biology, Transfection, Expression vector, Gene expression, Vector (molecular biology), Small interfering RNA, Gene