2002•PubMedRequires access
[Analysis with DNA chips of the changes of gene expressions in K562 cells in response to As2O3 treatment].
Chun-Qiong Feng, Wenli Ma, Ling Li, Yanbin Song, Rong Shi, Qinghua Wu, Qiuye Guo, Ji Zhu, Wenling Zheng
Open publisher page 1 citations
Abstract
OBJECTIVE: To investigate differential gene expression in apoptotic cells induced by As(2)O(3), and identify novel apoptosis-related genes. METHOD: Apoptosis of K562 cells cultured in RPMI 1640 medium supplemented with 10 % calf serum was induced by As(2)O(3). Total RNA of the apoptotic and normal cells were then extracted, purified and subject to reverse transcription into first-strand cDNA, labeled with Cy3/Cy5. Placenta DNA microarrays containing 348 DNA fragments were used to analyze the changes in gene expressions in the cells treated with As(2)O(3). RESULT: Eleven differentially expressed genes were identified in the apoptotic cells in comparison with the normal cells, 3 of which were associated with apoptosis, while the others were related to cell growth and proliferation. CONCLUSION: The placenta DNA microarrays we constructed may well apply to the analysis of the differentially expressed genes.