2011•Zhongguo xin yao zazhiRequires access
Gene expression profiles of rifampicin-injured rat liver using cDNA micoarray
Lishi Zhang
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Abstract
Objective:To investigate the gene expression profiles of rat liver tissue injured by rifampicin(RFP) using cDNA microarrays.Methods:Wistar rats were divided into 2 groups(10 in each group),and administrated(ig) with 100mg·kg-1 RFP or equal-volume saline for 14d,respectively.The gene expression profiles were detected by cDNA microarrays.The differentially expressed genes significantly correlated with liver injury were screened and analyzed.The mechanisms of liver injury caused by RFP were specifically analyzed at level of gene expression based on the biological functions of those differentially expressed genes.Results:Totally 19 differentially expressed genes were found in the rats administrated RFP.Among them,11 genes were up-regulated,while the other 8 genes were down-regulated.These differentially expressed genes were functionally related to the changes in CYP450-related genes,and the genes relevant to oxidation stress and special immune.Conclusion:RFP can cause remarkable changes in the gene expression profiles in rat liver cells,which is important for further elucidating the mechanisms of this type of liver injury.