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[Effect of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro].

Yang Song, Kedi Yang

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Abstract

OBJECTIVE: To study the effects of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro. METHODS: After separating Sertoli cells from testicular tissue of rats, we cultured these cells for 3 days and then added different doses of p, p'-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR. RESULTS: DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p, p'-DDE. CONCLUSION: p, p'-DDE could induce DNA damage and FasL gene expression of Sertoli cells of rat testis. p, p'-DDE might induce the Sertoli cell damage and destroy the dynamic process of spermatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism.

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What this paper is about

OBJECTIVE: To study the effects of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro. METHODS: After separating Sertoli cells from testicular tissue of rats, we cultured these cells for 3 days and then added different doses of p, p'-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR. RESULTS: DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p, p'-DDE. CONCLUSION: p, p'-DDE could induce DNA damage and FasL gene expression of Sertoli cells of rat testis. p, p'-DDE might induce the Sertoli cell damage and destroy the dynamic process of spermatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism.

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Available abstract

OBJECTIVE: To study the effects of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro. METHODS: After separating Sertoli cells from testicular tissue of rats, we cultured these cells for 3 days and then added different doses of p, p'-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR. RESULTS: DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p, p'-DDE. CONCLUSION: p, p'-DDE could induce DNA damage and FasL gene expression of Sertoli cells of rat testis. p, p'-DDE might induce the Sertoli cell damage and destroy the dynamic process of spermatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism.

Key concepts: Sertoli cell, Fas ligand, Gene expression, DNA damage, Biology, Gene, Molecular biology, In vitro

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[Effect of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro]. — Research Paper | ScholarLens