ScholarLens Back to search
2004•Journal of environmental healthRequires access

The Effect of p,p'-DDE on Transferrin in Sertoli Cells of Rat Testis in Vitro

Shihai Liu

Open publisher page 0 citations

Abstract

Objective To explore the effect of p,p'-DDE on transferrin expression in Sertoli cells of rat testis in vitro. Methods The effects of p,p'-DDE on expression of mRNA of transferrin gene in primary cultured Sertoli cells exposed to p,p'-DDE for 24 h was examined by one-step RT-PCR methods. Results The transferrin mRNA levels were decreased with the increases of p,p'-DDE concentration in Sertoli cell cultures,and there was a significant dose-effect relationship(P0.05). Conclusion p,p'-DDE could inhibit the translation of transferrin in rat Sertoli cells cultured in vitro.

About this research paper

What this paper is about

Objective To explore the effect of p,p'-DDE on transferrin expression in Sertoli cells of rat testis in vitro. Methods The effects of p,p'-DDE on expression of mRNA of transferrin gene in primary cultured Sertoli cells exposed to p,p'-DDE for 24 h was examined by one-step RT-PCR methods. Results The transferrin mRNA levels were decreased with the increases of p,p'-DDE concentration in Sertoli cell cultures,and there was a significant dose-effect relationship(P0.05). Conclusion p,p'-DDE could inhibit the translation of transferrin in rat Sertoli cells cultured in vitro.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the effect of p,p'-DDE on transferrin expression in Sertoli cells of rat testis in vitro. Methods The effects of p,p'-DDE on expression of mRNA of transferrin gene in primary cultured Sertoli cells exposed to p,p'-DDE for 24 h was examined by one-step RT-PCR methods. Results The transferrin mRNA levels were decreased with the increases of p,p'-DDE concentration in Sertoli cell cultures,and there was a significant dose-effect relationship(P0.05). Conclusion p,p'-DDE could inhibit the translation of transferrin in rat Sertoli cells cultured in vitro.

Key concepts: Sertoli cell, Transferrin, In vitro, Messenger RNA, Internal medicine, Endocrinology, Biology, Andrology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The Effect of p,p'-DDE on Transferrin in Sertoli Cells of Rat Testis in Vitro — Research Paper | ScholarLens