2006•PubMedRequires access

[Inhibition of the expression of p42MAPK in HeLa cell line by RNA interference].

Chen Huang, Liying Liu, Tusheng Song, Lei Ni, Li-ping Song, Jing-song Hu, Xiaoge Zhao, Lüsheng Si

Abstract

OBJECTIVE: To screen for siRNAs that inhibit the expression of p42(MAPK) in HeLa cell line. METHODS: Three p42(MAPK) siRNAs and one random siRNA were synthesized using Silencer siRNA Construction Kit, and labeled with Cy-3 for measurement of transfection effect. SiRNAs were transfected into HeLa cells by Lipofectamin 2000. The expression of p42(MAPK) was analyzed by Western blot. The biological effect of siRNAs on HeLa cell growth was monitored by MTT and flow cytometry. RESULTS: Two siRNAs (siRNA-2 and siRNA-3) among three tested were identified to be able to downregulate the p42(MAPK) expression. A concurrent growth retardation of HeLa cell line was observed in comparison with the control. CONCLUSION: Inhibition of p42(MAPK) expression with siRNA technique can inhibit the proliferation of HeLa cells.

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Key concepts: HeLa, Small interfering RNA, Transfection, MAPK/ERK pathway, RNA interference, Molecular biology, Cell culture, Cell biology