[Knockdown of Polo-like kinase 1 (PLK1) inhibits the growth of cervical cancer HeLa cells].

Objective To investigate the effect of knockdown of Polo-like kinase 1 (PLK1) on the growth of HeLa cells. Methods Flow cytometry was used to evaluate the delivery efficiency of siGFP by LAH4-L1 vector. Reverse transcription PCR was used to detect the mRNA level of PLK1. Western blot analysis was performed to detect the level of PLK1 protein. And CCK-8 assay was used to detect the viability and growth of HeLa cells. Results About 70% gene and protein silence was achieved in HeLa cells after LAH4-L1-siPLK1 nanocomplexes transfection, and the proliferation of HeLa cells was significantly inhibited. Besides, the high delivery efficiency of LAH4-L1 could maintain at a stable level. Conclusion Knockdown of PLK1 in HeLa cells can inhibit the growth of HeLa cells. LAH4-L1 is a good gene delivery vector.