2012•PubMedRequires access

[Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro].

Yu-ping Zhu, Dechuan Li, Haiyang Feng, Yong Liu, Jun Qian, Yinbo Chen, Yun Liang Gao

Abstract

OBJECTIVE: To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro. METHODS: A recombinant Bcl-xl adenovirus was constructed, amplified, and purified. The effect on mRNA expression of Bcl-XL was assessed by RT-PCR, and the effect on apoptosis-induction of colon cancer(Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay. RESULTS: RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells. Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP (P<0.05). Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dose-dependent manner (P<0.05). Ad/Bcl-XL shRNA showed no effect on normal human fibroblast. CONCLUSION: Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.

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Key concepts: Small hairpin RNA, Bcl-xL, Clonogenic assay, Apoptosis, Molecular biology, MTT assay, Medicine, In vitro