Antitumor activity of PB-LY and its mechanism in vitro

Aim To determine the antitumor activity of PB-LY and investigate its anticancer mechanism in vitro.Methods MTT and cell growth curve were used to study antitumor activity of PB-LY.Morphological study,DNA gel electrophoresis and flow cytometric analysis were used to determine tumor cell apoptosis induced by PB-LY.RT-PCR investigated the expression of Caspase-3 mRNA,Bax mRNA and Bcl-2 mRNA in Hela cells exposed to PB-LY.Results MTT showed that IC50 was 1.35 μmol·L-1～3.68 μmol·L-1.After tumor cells were treated with PB-LY,morphological characteristic of apoptosis was detected.Agarose gel electrophoresis showed typical DNA ladder.Flow cytometric analysis showed apoptotic Sub-Gl peak.RT-PCR showed that Caspase-3 mRNA and Bax mRNA levels increased,meanwhile the expression of Bcl-2 mRNA decreased.Conclusion PB-LY exerted obvious anticancer activity,which induced apoptosis by upregulating Caspase-3 and Bax,and downregu1ating Bcl-2.