[Mechanism of protective effect of amphiphilic compounds during hypertonic hemolysis of erythrocytes].

Antihemolytic effect of various amphiphilic compounds under conditions of red blood cell hypertonic hemolysis at different temperatures (0 and 37 degrees C) and cell exposure to diamide was investigated. The level of maximum antihemolytic activity and values of efficient concentrations for all studied substances were lower at 0 degrees C if compared with 37 degrees C. Exposure of erythrocytes to diamide (5 and 10 mmol/l) did not change cell sensibility to hypertonic medium. There has been demonstrated a temperature-dependent decrease in the efficiency of studiedsubstances under hypertonic hemolysis of erythrocytes exposed to 10 mmol/l diamide. Found reduction in efficiency of amphiphiles at low temperature and at high concentration of diamide was probably caused by similar changes of structural and dynamic state of erythrocyte membrane.