EFFECT OF PHENYLHYDRAZINE ON HYPERTONIC STRESS OF MAMMALIAN ERYTHROCYTES AND ANITHEMOLYTIC ACTIVITY OF AMPHIPHILIC COMPOUNDS

In the research work the initial level of erythrocyte damage of the investigated mammals has been shown to be significantly differed under hypertonic conditions. The level of human erythrocyte lysis in 4.0 mol/l NaCl at 37 °C made 90 %, for bovine cells it was 80 % and 60 % for equine ones. During the medium temperature decrease down to 0 °C the resistance of human, bovine and equine erythrocytes did not rise significantly. It has been demonstrated that sensitivity of mammalian erythrocytes to hypertonic stress at 37 °C after treatment with phenylhydrazine depended on the species of cells. Herewith the sensitivity of modified bovine and human erythrocytes was reduced and the equine ones increased. Sensitivity of rabbit erythrocytes after modification did not change statistically and significantly. Under hypertonic stress at temperature of 0 °C the rise in the damage rate of modified erythrocytes of all the investigated mammal species was observed. Trifluoperazine and dodecyl-b,D-maltoside has been established to manifest a high protective effect under hypertonic stress of erythrocytes for all the studied mammal species, excluding the rabbit erythrocytes. Trifluoperazine has a somewhat higher anithemolytic activity (about 80–90 %) if compared with dodecyl-b,D-maltoside (70 %). When decreasing in experiment the temperature down to 0 °C the antihemolytic activity of trifluoperazine and dodecyl-b,D-maltoside was strongly decreased. It has been shown that under hypertonic stress antihemolytic activity of amphiphilic compounds after modification of mammalian erythrocytes with phenylhydrazine was strongly decreased.