[Effects of different means of fluid resuscitation on the activity of nuclear factor-KappaB in peripheral blood mononuclear cells in rats with hemorrhagic shock].

OBJECTIVE: To observe the effects of different means of fluid resuscitation on the activity of nuclear factor-KappaB (NF-KappaB) in peripheral blood mononuclear cells (PBMC) in rats with hemorrhagic shock. METHODS: Rat models of hemorrhagic shock were reproduced using the modified Capone method. Thirty-two SD rats were randomly divided into four groups: control group, no fluid resuscitation group, limited fluid resuscitation group, large quantity fluid rapid resuscitation group (each n=8). The survival time and survival rate of each group were compared. The activities of NF-KappaB in PBMC were assessed with enzyme-linked immunosorbent assay (ELISA). RESULTS: The survival time of limited fluid resuscitation group was longer than that of large quantity fluid rapid resuscitation group and that of no fluid resuscitation group (both P<0.05). The 72-hour survival rate in limited fluid resuscitation group was higher than that in large quantity fluid rapid resuscitation group and also in no fluid resuscitation group, while lower than that of control group (all P<0.05). The activities of NF-KappaB in PBMC increased significantly 60 and 120 minutes after injury compared with those before injury except control group, that of 120 minutes was higher than 60 minutes (all P<0.05). The activities of NF-KappaB in limited fluid resuscitation group was lower than those in large quantity fluid rapid resuscitation group and also in no fluid resuscitation group (all P<0.05). The activities of NF-KappaB in the died rats were higher than those of the survivors 60 and 120 minutes after injury (both P<0.05). CONCLUSION: The results of our study demonstrate that the limited fluid resuscitation during hemorrhagic shock could raise the 72-hour survival rate and prolong survival time. The poor prognosis of hemorrhagic shock was associated with high NF-KappaB activity in PBMC, which could be lowered with limited fluid resuscitation.