The experimental study on the protective effect of limited fluid resuscitation on rats with uncontrolled hemorrhagic shock

Objective To investigate the protective effect of the early limited fluid resuscitation rats with severe uncontrolled hemorrhagic shock.Methods The model rats were randomly divided into four groups by the different fluid resuscitation means: the sham surgery group(SS)、no fluid resuscitation group(NF)、aggressive fluid resuscitation group(AR) and limited fluid resuscitation group(LR).The value of serum ALT of four rats in every group at 30min,90min,150min,270min,510min were detected,the hepatic tissue's pathologic changes was detected by hematoxylin and eosin(HE) dyeing.The loss volume of rat's tail continuous blood,the resuscitation fluid volume,the survival time and the survival rate of the rats were analysied.Results During the experiment,the loss volume of tail continuous blood of LR group of rats is lower than NF and AR group(P0.05),and the resuscitation fluid volume for LR group of rats is also lower than NF and AR group(P0.01).After treatment with different fluid resuscitation in traumatic emergency phrase,the value of ALT in NF、AR、LR group rat's was continuously increased with highest degree in NF group and lowest degree in LR group.The hepatic tissue's pathologic change of NF and AR group's rat was severer than that of LR group during the whole process.The rat's survival time of LR group was significant longer than that of NF and AR group(P0.01),and the survival rate of LR group in every monitoring time point,such as 150min、270min、510min、72h,was significant higher than that of NF and AR group.Conclusion After severe traumatic uncontrolled hemorrhagic shock,the earlier limited fluid resuscitation is effective to reduce the loss volume of tail continuous blood and the resuscitation fluid volume,to protect hepatic function,to increase the survival time and the survival rate of rats,which may be an ideal curative method for severe traumatic uncontrolled hemorrhagic shock.In clinical.