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Radioimmunoassay of porcine growth hormone -- methodological studies.

Gisela Tegeler, B Schülke

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Abstract

A double antibody radioimmunoassay for immunoreactive growth hormone in porcine plasma has been evaluated on the basis of dilution and recovery experiments as well as by examination of accuracy and reproducibility. The sensitivity was better than 0.10 ng/ml. The cumulated within-assay coefficient of variation was between 2--4% and the cumulated between-assay coefficient of variation was 9.6%. The normal fasting values correspond well to those of other investigators.

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What this paper is about

A double antibody radioimmunoassay for immunoreactive growth hormone in porcine plasma has been evaluated on the basis of dilution and recovery experiments as well as by examination of accuracy and reproducibility. The sensitivity was better than 0.10 ng/ml. The cumulated within-assay coefficient of variation was between 2--4% and the cumulated between-assay coefficient of variation was 9.6%. The normal fasting values correspond well to those of other investigators.

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Available abstract

A double antibody radioimmunoassay for immunoreactive growth hormone in porcine plasma has been evaluated on the basis of dilution and recovery experiments as well as by examination of accuracy and reproducibility. The sensitivity was better than 0.10 ng/ml. The cumulated within-assay coefficient of variation was between 2--4% and the cumulated between-assay coefficient of variation was 9.6%. The normal fasting values correspond well to those of other investigators.

Key concepts: Radioimmunoassay, Coefficient of variation, Reproducibility, Growth hormone, Dilution, Serial dilution, Chromatography, Internal medicine

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