[Consecutive spectrophotometric determinations in one cuvette: a new approach for checking the accuracy of single, profile and emergency determination (author's transl)].

A cost effective method for emergency and single assays is presented. By this method, a serial (consecutive) determination of reagent blank, sample, standard, and control serum is performed in one single cuvette and with only one pipetting of reagent. Compared to a sequential determination of sample, standard, and control serum, this is specially economical with regard to reagents and cuvettes. A particular application is seen in the field of prefilled cuvette tests, where the consecutive method allows analysis in the sample and in the control serum, and determination of the required standard absorption in a single test. The results found in the determination of glucose and of aspartate aminotransferase show that the consecutive measuring principle is feasible and even results in a more expressive control of accuracy. If the value found in the assay exceeds the measuring range due to too high a substance concentration or activity, or for photometry reasons, it is immediately seen from the incorrect value for the control serum. The same is not possible in the conventional technique, as it uses two separate tests for the sample and for the control serum.