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[Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm].

Ying Zhan, Ya-Nan Zhao, Zonglin Li, Li Li, Qian Song

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Abstract

OBJECTIVES: To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome. METHODS: Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe. RESULTS: 1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01). CONCLUSIONS: KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.

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What this paper is about

OBJECTIVES: To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome. METHODS: Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe. RESULTS: 1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01). CONCLUSIONS: KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.

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Available abstract

OBJECTIVES: To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome. METHODS: Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe. RESULTS: 1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01). CONCLUSIONS: KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.

Key concepts: Acrosin, Acrosome, Sperm, Acrosome reaction, Chemistry, Andrology, Capacitation, Staining

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[Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm]. — Research Paper | ScholarLens