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[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell].

Lingyun Dai, X Zhang, Hui Hu

Abstract

OBJECTIVE: To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell. METHODS: An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine. RESULTS: Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line. CONCLUSION: PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.

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Key concepts: Proliferating cell nuclear antigen, Lipofectamine, Transfection, Antisense RNA, Molecular biology, RNA, Biology, Cell growth