2011•PubMedRequires access
[Establishment of B lymphoblastoid cell lines of Miao pedigree with Bardet-Biedl syndrome].
Tao Shen, Tao Shou, Keqin Lin, Wen Yi, Ying-kun Hua, Hong Dong, Jian-Mei Gao, Jinping Zhang, Linping Wang, Jin-li Wang, Li Li, Xinmin Yan
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Abstract
OBJECTIVE: To establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome (BBS), in order to provide a long-term source of material for research. METHODS: With Epstein-Barr virus transformation of B cells and addition of cyclosporine A to inhibit the activity of T cells, fresh anticoagulated blood samples with heparin were collected from 12 members of the core pedigree, and were used to establish the immortalized lymphoblastoid cell lines of B lymphocytes. RESULTS: Twelve immortalized lymphoblastoid cell lines of the core BBS pedigree were obtained successfully. CONCLUSION: The immortalized B lymphoblastoid cell lines of the Miao pedigree with BBS can preserve the whole genome information and provide long-term research materials for BBS study.