Establishment of Immortalized Lymphoblastoid Cell Bank of Hereditary Gingival Fibromatosis Pedigrees and Their Karyotype Analysis

Objective:To establish immortal lymphoblastoid cell lines of hereditary gingival fibromatosis(HGF)and analyse the feasibility of these cell lines used as the specimens in research of cytogenetics.Methods:With CyA and Epstein-barr virus transformation,peripheral blood and frozen leukocyte from five autosomal dominant HGF pedigrees were collected and used respectively to establish the immortalized lymphoblastoid cell lines of B-lymphocytes.Chromosomal karyotype of lymphocytes cell lines were analysed subsequently.Results:Total 32 immortalized lymphoblastoid cell lines were obtained successfully.No chromosomal abnormalities were found in the cultured cells until passages 20 and 25,in which variation in chromosome number and structure were detected.Conclusion:The established cell bank of HGF in our study can preserve the whole genome information and provide long term DNA samples in the future genetics and molecular study.