2001•PubMedRequires access
[Differential display of vincristine-resistant proteins in gastric cancer cell line SGC7901].
Xiaoming Wang, Y Shi, Y. Zhao
Open publisher page 2 citations
Abstract
OBJECTIVE: To find out new multidrug-resistant proteins in gastric cancer cells SGC7901 and to explain the new multidrug-resistant mechanism of gastric cancer cells. METHODS: Two-dimensional gel electrophoresis was used with immobilized pH gradients (IPG) to compare the differential expression of multidrug-resistant proteins in gastric cancer cells SGC7901 and Vincristine-resistant SGC7901 cells (SGC7901/VCR) induced by vincristine sulfate. 2-D gels were used to silver stain the protein. RESULTS: Approximately, 680 protein spots were identified in each of the 2-D gel patterns by silver stain. With most of them showing no difference in composition, shape or density, twenty-five proteins were found to differ in quantity (6 higher in SGC7901/VCR cells; 19 higher in 7901 cells). Five proteins were seen to be unique in one region or the others (3 in SGC7901/VCR cells, 2 in 7901 cells). The coordinate position of 30 protein spots in the 2-D gels were listed. CONCLUSION: The results suggest that these differential proteins be related to the vincristine-resistant mechanism in human gastric cancer cell line SGC7901/VCR.