2010•PubMedRequires access

Staining with highly sensitive Coomassie brilliant blue SeePico™ Stain after Flamingo™ fluorescent gel stain is useful for cancer proteomic analysis by means of two-dimensional gel electrophoresis.

Yasuhiro Kuramitsu, Eiko Hayashi, Futoshi Okada, Xiulian Zhang, Toshiyuki Tanaka, Yoshiya Ueyama, Kazuyuki Nakamura

Abstract

Highly sensitive Coomassie brilliant blue SeePico™ Stain was applied for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). After staining with Flamingo™ Fluorescent Gel Stain, the images of the protein spots were analyzed, and 424 protein spots were detected. After washing with Milli-Q water three times, the gels were re-stained with SeePico™ Stain and the images of the protein spots were analyzed; 272 spots were detected. To assess whether SeePico™ Stain alters MS analysis, a spot was picked up and was analyzed by LC-MS/MS. The MS analysis showed good protein identification. These results show a possible role for SeePico™ Stain in cancer proteomics using 2-DE and MS.

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Key concepts: Stain, Coomassie Brilliant Blue, Staining, Two-dimensional gel electrophoresis, Gel electrophoresis, Chromatography, Mass spectrometry, Chemistry