[P-31 MR spectroscopy of excised pig liver].
Chikashi Negishi
Abstract
Chikashi Negishi
Abstract
This study used P-31 MR spectroscopy (P-31 MRS) to evaluate the viability of a pig liver preserved with perfusate. Pig liver was excised and preserved in cooled Euro-Collins solution after canulation of the portal vein and perfusion with the solution. P-31 MRS was performed on a 1.5 Tesla machine. P-31 MRS showed that the beta-peak of ATP in the preserved pig liver diminished within two hours. P-31 MRS of pig liver with perfusion of cooled and oxygenated Euro-Collins solution revealed a significant increase in the beta-peak in the pig liver preserved 7 hr compared with that preserved for a longer time. Assessing the beta-peak of ATP by P-31 MRS with cooled and oxygenated Euro-Collins solution is valuable to evaluate the viability of preserved pig liver.
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This study used P-31 MR spectroscopy (P-31 MRS) to evaluate the viability of a pig liver preserved with perfusate. Pig liver was excised and preserved in cooled Euro-Collins solution after canulation of the portal vein and perfusion with the solution. P-31 MRS was performed on a 1.5 Tesla machine. P-31 MRS showed that the beta-peak of ATP in the preserved pig liver diminished within two hours. P-31 MRS of pig liver with perfusion of cooled and oxygenated Euro-Collins solution revealed a significant increase in the beta-peak in the pig liver preserved 7 hr compared with that preserved for a longer time. Assessing the beta-peak of ATP by P-31 MRS with cooled and oxygenated Euro-Collins solution is valuable to evaluate the viability of preserved pig liver.
Key concepts: Pig liver, Perfusion, Portal vein, Miniature pig, Chemistry, Liver perfusion, Liver tissue, Nuclear medicine