1993•The International Journal of Artificial OrgansRequires access
Nonenzymatic versus Enzymatic Hepatocyte Isolation from Pig Livers for Larger Scale Investigations of Liver Cell Perfusion Systems
J. Gerlach, Jochen Brombacher, J.M. Courtney, P. Neuhaus
Open publisher page 26 citations
Abstract
A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.