1996•PubMedRequires access
[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization].
Gurskaia Ng, Dmitry A. Shagin, Luk'ianov Ka, Vagner Ll, Shtutman Ms, Musatkina Ea, Moinova Ev, Tatosian Ag, Luk'ianov Sa, Sverdlov Ed
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Abstract
Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.