2002•PubMedRequires access
[Molecular cloning of genes differentially expressed in human lung squamous cell carcinoma using suppression subtractive hybridization].
Chenyang Shen, Jun Liu, Danlei Wang, Hui Zhao, Guanchao Jiang, Jun Wang, Guoliang Zhang
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Abstract
BACKGROUND: To clone and identify genes differentially expressed in human lung squamous cell carcinoma (LSCC). METHODS: A subtracted cDNA library of human LSCC was constructed by suppression subtracted hybridization (SSH) method. After screening, the subtracted library clones representing mRNAs that were truly differentially expressed in LSCC but not in its adjacent non cancerous tissues were selected to identify by RT-PCR and DNA sequencing were performed. Nucleic acid homology searches were performed using the BLAST program. RESULTS: By this technique, 10 differentially expressed gene cDNA fragments of LSSC were obtained. Two were novel and eight were already known genes. CONCLUSIONS: SSH is a useful technique with high sensitivity for the detection of differential genes expression in LSCC and an effective method to clone novel genes.