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Regeneration of Shoots on Root Explants of Flax

ZHAN XIANG-CAN, David A. Jones, A. Kerr

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Abstract

Regeneration of shoots from adventitious root explants of flax was achieved for two of five cultivars tested. Root explants of the cultivar Bombay regenerated buds on MS medium with various supplements, the best combination being 002 mg I−1 NAA, 1 mg I− 6-BA, 20 mg I− adenine and 500 mg I− cefotaxime. Adenine, in the presence of 6-BA, and cefotaxime, in the presence of both 6-BA and adenine, were found to stimulate bud initiation, but the most important factor influencing bud regeneration from flax roots was genotype

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What this paper is about

Regeneration of shoots from adventitious root explants of flax was achieved for two of five cultivars tested. Root explants of the cultivar Bombay regenerated buds on MS medium with various supplements, the best combination being 002 mg I−1 NAA, 1 mg I− 6-BA, 20 mg I− adenine and 500 mg I− cefotaxime. Adenine, in the presence of 6-BA, and cefotaxime, in the presence of both 6-BA and adenine, were found to stimulate bud initiation, but the most important factor influencing bud regeneration from flax roots was genotype

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Available abstract

Regeneration of shoots from adventitious root explants of flax was achieved for two of five cultivars tested. Root explants of the cultivar Bombay regenerated buds on MS medium with various supplements, the best combination being 002 mg I−1 NAA, 1 mg I− 6-BA, 20 mg I− adenine and 500 mg I− cefotaxime. Adenine, in the presence of 6-BA, and cefotaxime, in the presence of both 6-BA and adenine, were found to stimulate bud initiation, but the most important factor influencing bud regeneration from flax roots was genotype

Key concepts: Explant culture, Biology, Shoot, Cultivar, Regeneration (biology), Botany, Murashige and Skoog medium, In vitro

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