2011•Agricultural Science and Technology HunanRequires access

Optimization of genetic transformation system of tobacco K326 mediated by Agrobacterium.

Qin Zhao

Abstract

[Objective]The aim was to optimize genetic transformation system in tobacco K326 mediated by Agrobacterium.[Method]The leaf of tobacco aseptic seedling was taken as explants to study the optimization of Agrobacterium-mediated genetic transformation system.[Result] The highest transformation efficiency was obtained when the explants were pre-cultured in the medium of MS + 2 mg/L 6-BA + 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101(OD600 =0.6) for 5 min.The PCR detection proved that npt II gene had been integrated into the regenerated tobacco plants.[Conclusion]A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.

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Key concepts: Agrobacterium, Transformation (genetics), Explant culture, Biology, Callus, Transformation efficiency, Horticulture, Botany