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Optimization of Agrobacterium-mediated K326 Genetic Transformation System

Qin Zhao

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Abstract

[Objective] The aim was to optimize tobacco K326 genetic transformation system mediated by Agrobacterium.[Method] The leaf of tobacco aseptic seedling was taken as explants to study the optimization of K326 genetic transformation system via Agrobacterium.[Result] The highest transformation efficiency was obtained when the explants was pre-cultured in the medium of MS + 2 mg/L 6-BA + 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101(OD600 = 0.6) for 5 min.The PCR detection proved that NPTII gene had been integrated into the regenerated tobacco plants.[Conclusion] A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.

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What this paper is about

[Objective] The aim was to optimize tobacco K326 genetic transformation system mediated by Agrobacterium.[Method] The leaf of tobacco aseptic seedling was taken as explants to study the optimization of K326 genetic transformation system via Agrobacterium.[Result] The highest transformation efficiency was obtained when the explants was pre-cultured in the medium of MS + 2 mg/L 6-BA + 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101(OD600 = 0.6) for 5 min.The PCR detection proved that NPTII gene had been integrated into the regenerated tobacco plants.[Conclusion] A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.

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Available abstract

[Objective] The aim was to optimize tobacco K326 genetic transformation system mediated by Agrobacterium.[Method] The leaf of tobacco aseptic seedling was taken as explants to study the optimization of K326 genetic transformation system via Agrobacterium.[Result] The highest transformation efficiency was obtained when the explants was pre-cultured in the medium of MS + 2 mg/L 6-BA + 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101(OD600 = 0.6) for 5 min.The PCR detection proved that NPTII gene had been integrated into the regenerated tobacco plants.[Conclusion] A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.

Key concepts: Agrobacterium, Transformation (genetics), Explant culture, Biology, Callus, Transformation efficiency, Horticulture, Gene

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