Establishment of five cell lines from normal adult rat livers in vitro.

Cells isolated by trypsin were cultured from the liver of normal rats of the Donryu strain. Cells were collected from five animals of different age groups from 40 days to 12 months. All five cell collections produced different cell lines according to animal age. The establishment of these cell lines was not dependent upon the method of perfusion by Ca++-and Mg++-free phosphate buffered saline. Minor morphological variations were observed among the five lines. All cell lines showed epithelial figures under phase contrast microscopy. An increase in cells with trisomy of No. 1 chromosome was found after two to three months of cell culture.