Systematic Analysis of Steroids. I. Systematic Analysis of Steroid Hormones by Thin Layer Chromatography.

Systematic and simultaneous analysis of twenty-six steroid hormones was carried out by thin layer chromatography. Twenty-eight solvent systems consisting of various organic solvents in various proportions were used as the mobile phase. The interrelationship among chemical structure and adsorptivity onto silica gel of steroid hormones is discussed. Characteristic color reaction of steroid hormones and four coloring reagents was examined.