Effects of lancemaside D on time effect of proliferation and cell cycle of HepG-2 cells

Objective To investigate the effect of lancemaside D on the time effects of cell proliferation and cell cycle of HepG-2 cells and explore the anticancer active ingredients and mechanisms of codonopsis lanceolata.Methods The HepG-2 cells were treated with different concentrations(5,10,15,20 and 25 mg·L-1) of lancemaside D in vitro,meanwhile control group was set up.The proliferation inhibition of HepG-2 cells was detected by MTT assay,and the change of cell cycle of HepG-2 cells was determined by flow cytometry.Results The results showed that the inhibitory rate of cell proliferation of HepG-2 cells were significantly higher than that in control group(P0.01) after treated with 25 mg·L-1 lancemaside D for 12,24,48 and 72 h;the cell numbers in lancemaside D groups were significantly smaller than that in control group after treated with lancemaside D for 48,72,96 and 120 h(P0.05),among 5,15,20 and 25 mg·L-1groups there were significant differences(all P0.05);the HepG-2 cell proliferation speed was delated with the increasing of lancemaside D concentration.The cell cycle detection results showed that in the lancemaside D concentration range of 10-25 mg·L-1,the percentages of HepG-2 cells at G0/G1 phase were 55.97%±0.42%,57.16%±0.13%,57.48%±0.15% and 60.39%±0.32%,respectively,they were significantly higher than that in control group(53.22%±0.28%)(P0.05).In the same concentration range of lancemaside D,the percentages of HepG-2 cells at S phage were 27.47%±1.42%,26.15%±2.71%,26.34%±0.67% and 24.81%±0.46%,they were significantly lower than that in control group(33.47%±0.25%)(P0.05),which suggested a G0/G1 cell cycle arresting in lancemaside D group.Conclusion Lancemaside D strongly inhibits the growth of HepG-2 cells in vitro and furthermore cause cell DNA metabolism disturbance.