Effect of raddeanin A on the proliferation of human hepatic cancer cells HepG_2

Objective To investigate the effect of raddeanin A on the proliferation of human hepatic cancer cells HepG2. Methods Human hepatic cancer cells HepG2 were co-cultured with different concentrations of raddeanin A,the effect of raddeanin A on the growth of HepG2 cells was examined using MTT assay and colonogenic assay. Results Raddeanin A against the HepG2 cells with IC50 of 8.94 μg/mL. HepG2 cells were co-cultured with 5,10,20,and 40 μg/mL raddeanin A for 72 h,against the cells proliferation with inhibitory rate of (24.81±0.55)%,(39.17±1.30)%,(62.45±7.56)%,and (79.93±5.48)% at 24 h; (35.67±0.81)%,(49.73±9.18)%,(71.62±1.03)%,and (87.06±1.84)% at 48 h; (42.52±1.31)%,(59.75±7.47)%,(78.85±3.15)%,and (91.36±0.84)% at 72 h,respectively,compared with control group. Cisplatin showed the inhibitory rate of (2.60±0.53)%,(60.55±5.66)%,and (82.61±8.95)%,respectively. Each dose group in comparison with the vehiculum group,were significant differences in various time point (P0.001). In comparison with cisplatin group,raddeanin A inhibited more rapidly the proliferation of HepG2 cells at 24 h (P0.001). Other concentrations of the compound except at 40 μg/mL,have no difference compared with cisplatin,at different times point. Effect of raddeanin A 40 μg/mL against HepG2 cells proliferation was still stronger than cisplatin (P0.01) at different times point. Colonogenic assay demonstrated that the colony-forming rate were 15.73%,7.2%,and 3.33%,respectively,viz colony-forming inhibitory rate were (66.75±1.66)%,(84.25±3.11)%,and (92.55±2.33)%,respectively,compared with the control group were significantly different (P0.001). Conclusion Raddeanin A showed significanly inhibitory on proliferation of HepG2 cells in vitro,and at certain range the inhibitory effects was depended on the medicine concentration and treating times.