Simultaneous Determination of the Content of Baicalin and Berberine Hydrochloride in Sanhuang Tablets by HPLC

Objective:To establish a HPLC method for simultaneous determination of baicalin and berberine hydrochloride in Sanhuang tablets.Methods:The determination was performed on Kromasil C18(250 mm × 4.6 mm,5 μm),and acetonitrile-0.1% H3PO4 which contained 1 g ·L-1 sodium dodecyl sulfate(45:55) was used as mobile phase.The detection wavelength was 265 nm,the flow rate was 1.0 mL ·min-1,and the column temperature was equal to room temperature.Results:It showed a good linear relationship between the peak areas and the contents of baicalin and berberine hydrochloride in the ranges of 0.086 00-4.320 and 0.102 0-1.533 μg,respectively.The contents of baicalin and berberine hydrochloride in the commercially available Sanhuang tablets were 0.490 0-20.53 and 2.81-5.12 mg,respectively.Conclusion:This method is simple for simultaneous determination of baicalin and berberine hydrochloride in Sanhuang tablets.The feeding supervision of Scutellaria extract in the production should be strengthened because the contents of baicalin from different manufactures vary significantly.