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2002•Zhongguo shengwuzhipinxue zazhiRequires access

Analysis of recombinant HIV-1 P24 antigen

Wei Chen, Kejin Chen, Zhu Hua-song, Mosong Yu

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Abstract

Objective To analyze the recombinant HIV-1 p24 antigen prepared by the authors and lay a foundation of developing diagnostic kit for HIV antibody. Methods National reference for HIV-Ab and normal human sera were detected by ELISA using recombinant p24 as coated antigen. Results The accuracy rates of recombinant p24 antigens used for detecting HIV-Ab positive and negative references were 95% (19/20) and 90%(18/20) respectively. However,no HIV-Ab was detected in 50 specimens of normal human sera. Conclusion The prepared recombinant p24 antigen showed high specificity and sensitivity and was suitable for developing diagnostic kit for HIV-Ab.

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What this paper is about

Objective To analyze the recombinant HIV-1 p24 antigen prepared by the authors and lay a foundation of developing diagnostic kit for HIV antibody. Methods National reference for HIV-Ab and normal human sera were detected by ELISA using recombinant p24 as coated antigen. Results The accuracy rates of recombinant p24 antigens used for detecting HIV-Ab positive and negative references were 95% (19/20) and 90%(18/20) respectively. However,no HIV-Ab was detected in 50 specimens of normal human sera. Conclusion The prepared recombinant p24 antigen showed high specificity and sensitivity and was suitable for developing diagnostic kit for HIV-Ab.

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Available abstract

Objective To analyze the recombinant HIV-1 p24 antigen prepared by the authors and lay a foundation of developing diagnostic kit for HIV antibody. Methods National reference for HIV-Ab and normal human sera were detected by ELISA using recombinant p24 as coated antigen. Results The accuracy rates of recombinant p24 antigens used for detecting HIV-Ab positive and negative references were 95% (19/20) and 90%(18/20) respectively. However,no HIV-Ab was detected in 50 specimens of normal human sera. Conclusion The prepared recombinant p24 antigen showed high specificity and sensitivity and was suitable for developing diagnostic kit for HIV-Ab.

Key concepts: Recombinant DNA, Antigen, Human immunodeficiency virus (HIV), Virology, Antibody, Medicine, Immunology, Biology

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