Susceptibility testing of Candida species to five antifungal drugs

Objective To study the in-vitro susceptibilities of Candida species collected from clinical samples to fluconazole, amphotericin B, itraconazole, 5-flucytosine and ketoconazole. Methods 108 isolates of Candida were tested for their minimun inhibitory concentration(MIC) to five antifungal agents by broth microdilution method using Sensititre YeastOne panels. Results In total, there are 8(7.4%), 15(13.9%) and 2(1.9%) isolates displaying resistant to fluconazole, itraconazole, 5-flucytosine respectively. Candida albicans are generally lowest MIC 90 of five drugs among all isolates. Candida glabrata isolates have significantly higher MIC to fluconazole, itraconazole and ketoconazole, comparing with all other Candida species, but still susceptible to amphotericin B and 5-flucytosine. The cross resistance in azoles is also discovered in Candida glabrata. Conclusions Candida species display different resistance to five antifungal drugs.