Measurement of the antifungal susceptibility of Candida spp. by broth microdilution method in vulvovaginal candidiasis

To measure the susceptibility of Candida spp. against antifungal agents in vulvovaginal candidiasis(VVC) cases, API 20 CAUX method was used to identify Candida species and according to the NCCLS M 27A scheme 126 strains were studied, including 120 isolates from the vaginal discharge of VVC patients, 5 reference strains and 1 quality control strain. The result showed that 105 of 120 isolates(87.5%) were Candida albicans, 6 of 120(7.5%)Candida glabrata,3 of 120(2.5%)Candida parapsilosis, 3 of 120 (2.5%)Candida tropicalis and 3 of 120(2.5%)Candida krusei. The mean MIC value(μg/mL)were: fluconazole 9.474±2.329, itraconazole 0.305±0.062, amphotericin B 0.599±0.062, ketoconazole 0.855±0.377, terbinafine 9.970±3.324, flucytosine 14.819±5.180, respectively. The result demonstrated high susceptibility of fluconazole, amphotericin B, itraconazole and ketoconazole, but lower sensitivity of terbinafine and flucytosine.The main species of yeast was still C.albicans.