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2011•Chinese Journal of ArteriosclerosisRequires access

Effect of Amlodipine on PDGF-B mRNA Expression in HUVEC-12 Endothelial Cells Induced by Oxidized Low Density Lipoprotein

He Jin

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Abstract

Aim To study the effect of amlodipine on platelet-derived growth factor-B(PDGF-B) mRNA expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein(ox-LDL) to explore the new antiatherosclerotic role of amlodipine.Methods HUVEC-12 endothelial cells were treated with 50 mg/L ox-LDL for 24 hours through the preexperiment.The experiment groups included 5 groups: control group,ox-LDL group,different concentration(0.1,1.0 and 10.0μmol/L) of amlodipine pretreated for 0.5 h and treated with 50 mg/L ox-LDL for 24 h group.RT-PCR was used to detect the mRNA expression of PDGF-B.Results RT-PCR analysis showed that with ox-LDL and amlodipine treatment,the mRNA expression of PDGF-B was greatly decreased in a concentration-dependent manner.Conclusion Ox-LDL can affect the mRNA expression of PDGF-B in the HUVEC-12 endothelial cells.Amlodipine can down-regulate the mRNA expression of PDGF-B.

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What this paper is about

Aim To study the effect of amlodipine on platelet-derived growth factor-B(PDGF-B) mRNA expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein(ox-LDL) to explore the new antiatherosclerotic role of amlodipine.Methods HUVEC-12 endothelial cells were treated with 50 mg/L ox-LDL for 24 hours through the preexperiment.The experiment groups included 5 groups: control group,ox-LDL group,different concentration(0.1,1.0 and 10.0μmol/L) of amlodipine pretreated for 0.5 h and treated with 50 mg/L ox-LDL for 24 h group.RT-PCR was used to detect the mRNA expression of PDGF-B.Results RT-PCR analysis showed that with ox-LDL and amlodipine treatment,the mRNA expression of PDGF-B was greatly decreased in a concentration-dependent manner.Conclusion Ox-LDL can affect the mRNA expression of PDGF-B in the HUVEC-12 endothelial cells.Amlodipine can down-regulate the mRNA expression of PDGF-B.

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Available abstract

Aim To study the effect of amlodipine on platelet-derived growth factor-B(PDGF-B) mRNA expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein(ox-LDL) to explore the new antiatherosclerotic role of amlodipine.Methods HUVEC-12 endothelial cells were treated with 50 mg/L ox-LDL for 24 hours through the preexperiment.The experiment groups included 5 groups: control group,ox-LDL group,different concentration(0.1,1.0 and 10.0μmol/L) of amlodipine pretreated for 0.5 h and treated with 50 mg/L ox-LDL for 24 h group.RT-PCR was used to detect the mRNA expression of PDGF-B.Results RT-PCR analysis showed that with ox-LDL and amlodipine treatment,the mRNA expression of PDGF-B was greatly decreased in a concentration-dependent manner.Conclusion Ox-LDL can affect the mRNA expression of PDGF-B in the HUVEC-12 endothelial cells.Amlodipine can down-regulate the mRNA expression of PDGF-B.

Key concepts: Amlodipine, Messenger RNA, Platelet-derived growth factor receptor, Chemistry, Low-density lipoprotein, Molecular biology, Growth factor, Platelet-derived growth factor

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Effect of Amlodipine on PDGF-B mRNA Expression in HUVEC-12 Endothelial Cells Induced by Oxidized Low Density Lipoprotein — Research Paper | ScholarLens