Expression of TLR2,TLR3,TLR5 and TLR9 in colon of ulcerative colitis mice

【Objective】To explore the role of Toll-like receptors(TLRs) family in ulcerative colitis(UC) pathogenesis by investigating the change of Toll-like receptor 2(TLR2),TLR3,TLR5,TLR9 over time in colon of ulcerative colitis mice.【Methods】60 BALB/c mice were randomly divided into 3 groups,Group 1 was untreated,Group 2 was feed with 5% dextran sodium sulfate(DSS) for 7 days,Group 3 was feed with 5% DSS for 14 days.The level of mRNA and protein of TLR2,TLR3,TLR5,TLR9 in colon was detected using RT-PCR and Western blotting.【Results】There was no expression of TLR2,TLR3,TLR5,TLR9 in colon of normal mice.After 7 days induced with DSS,the expression of TLR2,TLR9 was showed detectable in the colon,and their expression was continued increasing after induction for 14 days.The expression of TLR3 and TLR5 was not detected in three groups.【Conclusions】These results indicated TLR2,TLR9 may be involved in the pathogenesis of UC,while no clue showed TLR3,TLR5 played any roles in the pathogenesis of UC.