Preventive effects of TLR4 monoclonal antibodies on the gut mucosal cytokines(IL-17,IL-10 and TGF-β)expression in mice with DSS-induced acute ulcerative colitis

Objective To evaluate the preventive effects of toll like receptor 4 monoclonal antibodies(TLR4mAb) on inflammation,the gut mucosal cytokines(IL-17,IL-10 and TGF-β)expression in dextran sulfate sodium(DSS) induced acute ulcerative colitis(UC) in mice. Methods Thirty male BALB/c mice were randomly assigned into five groups from group A to group E: normal control group(A),UC model group(B),low dose,moderate dose and large dose intervention group(C,D,E).Group B to group E were only given 5.0%(wt/wt) DSS solution as drinking water during 7 days' observation as to induce acute intestinal inflammation,while the normal control was drinking distilled water freely.Group C,D,E recieved TLR4mAb injection every 48 hours intraperitoneally at the same time after the start of the DSS exposure.Daily disease activity index(DAI) and histopathological score(HPS) were evaluated.The mRNA expression of IL-17,IL-10 and TGF-β were measured by Real time-PCR. Results(1) Compared with the control group,the DAI and HPS in the UC group were markedly higher(P0.01).Compared with the UC group,the DAI and HPS in group C to E all decreased.(2) Compared with the UC group,the expression of three cytokine factors,such as IL-17,IL-10 and TGF-βin group C to E all decreased. Conclusions TLR4mAb can inhibit the excessive immune activatity of the intestinal tract by downregulating the expression of cytokines.