Experimental Study on Non-myeloablative Allogeneic Bone Marrow Transplantation for Treatment of Leukemia Mice

To explore the curative effects of non-myeloablative allogeneic bone marrow transplantation for treatment of leukemia mice. Six hundred and fifteen (H-2K) mice were loaded with L7212 cells and 2 days later underwent total body irradiation(TBI) 8.5 or 5Gy(60Co γ-ray) followed by allogeneic bone marrow transplantation(allo-BMT). The allografts consisted of 5×106 bone marrow cells mixed 1.5×107 spleen cells from BALB/C (H-2d) donor mice. Two days after allo-BMT, the recipient mice were given 200mg/kg of CTX. Afterwards mice of donor lymphocyte infusions(DLI) group received 5×106, 1×107, 2×107 spleen cells from donor mice. The engrafment of allograft, graft versus host disease(GVHD), the survival days and transplantation-related complications were observed. The nonmyeloablative conditioning could ensured the engraftment of allograft, the survival days of mice in nonmyeloablative allo-BMT group were 22.3±4.8, which was much longer than that of nonmyeloablative blank group 14.7±3.4 and myeloablative group 18.3±3.2(P0.05). There was difference between survival days of mice in DLI group and nonmyeloablative allo-BMT group (P0.05). The mice in DLI group had no obvious GVHD signs and histological changes. Furthermore the transplantation-related complications had reduced. [Conclusions] The potential GVL effects can be preserved in nonmyeloablative while the degree of GVHD is reduced, and the method of DLI after BMT can reinforce graft versus leukemia(GVL) effects while reduce transplantation-related complications.