Expressions of GST-π,TopoIIβ and MDR1 in ovarian epithelial carcinoma tissues and their chemotherapeutic responses

Objective To investigate expressions of Glutathione-s-transferase-π(GST-π),topoisomeraseⅡβ(Topo Ⅱβ)and multi-drug resistance gene 1(MDR1)in ovarian epithelial carcinoma tissues and their significances.Methods Reverse transcriptase polymerase chain reaction was used to detect expressions of GST-π mRNA,TopoⅡβ mRNA and MDR1mRNA in 60 cases with ovarian epithelial carcinoma and 60 cases with normal ovarian.The relations between the expression and the clinical-pathological characteristics as well as chemotherapeutic responses were discussed.Results GST-π mRNA and MDR1 mRNA were negative in normal ovarian tissue,while TopoⅡβ mRNA was postive.The positive rate of GST-π mRNA in ovarian epithelial carainoma tissues was 51.67%: there were no statistical significances among different pathological types and differential degrees(P0.05);positive rate of late period was higher than that of early period(P0.05).The positive rate of TopoⅡβ in ovarian epithelial carcinoma was 55%: there were no statistical significances among different pathological types and differential degrees(P0.05);positive rate of early period was higher that of late period(P0.05).The positive expression rate of MDR1 in ovarian epithelial carcinoma tissues was 35%,and a significant relationship was shown between its expression and pathologic differentiation degrees(P0.05),but there was no statistical differences among clinical stages.ConclusionsFor ovarian epithelial carcinoma patients,chemotherpeutic responses can be prediceted by detecting the expressions of GST-π,TopoⅡβ and MDR1.